Journal: Frontiers in Plant Science
Article Title: Wheat endosperm-specific transcription factor TaDOF6 enhances grain development by regulating TaSWEET13h expression and facilitating sugar and gibberellin transport
doi: 10.3389/fpls.2025.1608090
Figure Lengend Snippet: Structure and localization analysis of TaSWEET13h transporter. (A) TaSWEET13h protein sequence. Blue boxes and orange dots represent the transmembrane domain and MtN3_slv domains, respectively. (B) TMHMM analysis of the transmembrane domain profile of TaSWEET13h. (C) Cartoon image of the 3D structure of TaSWEET13h resolved by AlphaFold 2. (D) Subcellular localization of TaSWEET13h in wheat leaf protoplasts. The protoplasts transfected with the 16318h- GFP plasmid are used as a control, with mCherry fluorescence representing the marker of cytoplasmic membrane. Scale bar=5 μm.
Article Snippet: The TaDOF6 CDS was cloned into the Bam H I-digested 16318h- GFP vector (TaKaRa), provided by Dr. Yimiao Tang (Beijing Academy of Agriculture and Forestry Sciences).
Techniques: Sequencing, Transfection, Plasmid Preparation, Control, Fluorescence, Marker, Membrane